Type 2 Immunity by R. Lee Reinhardt

Author:R. Lee Reinhardt
Language: eng
Format: epub, pdf
Publisher: Springer New York, New York, NY

Fig. 2Imaging of IL-4 expressing and IL-4 producing T cells in the lymph node of N. brasiliensis-infected mice. IL44get or IL4KN2 mice were infected with 500 (L3) N. brasiliensis worms. The lung-draining lymph node was harvested and prepared for histology 9 days later. (a) Representative 50× images (5x objective) of the entire lymph node. Lymph nodes were stained for CD4 (red), GFP/IL-4 (green), and IgD (blue). A composite image is shown. A WT control for negative GFP staining is shown on the far right. (b) Representative 10× images of the lymph node showing a germinal center as negative IgD staining within the B-cell follicle. A WT control of GFP staining is shown on the right. (c) A 100× image (10x objective) of fresh-frozen IL4KN2 lymph node is shown. The lymph node was stained for CD4 (red), huCD2/IL-4 (green), and IgD (blue). A WT control for huCD2 staining is shown in a composite on the far right

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